New project in Nature Publishing Group from our research team.

In angiogenesis with concurrent inflammation, many pathways are activated, some linked to VEGF and others largely VEGF-independent. Pathways involving inflammatory mediators, chemokines, and micro-RNAs may play important roles in maintaining a pro-angiogenic environment or mediating angiogenic regression. Here, we describe a gene expression dataset to facilitate exploration of pro-angiogenic, pro-inflammatory, and remodelling/normalization-associated genes during both an active capillary sprouting phase, and in the restoration of an avascular phenotype. The dataset was generated by microarray analysis of the whole transcriptome in a rat model of suture-induced inflammatory corneal neovascularisation. Regions of active capillary sprout growth or regression in the cornea were harvested and total RNA extracted from four biological replicates per group. High quality RNA was obtained for gene expression analysis using microarrays. Fold change of selected genes was validated by qPCR, and protein expression was evaluated by immunohistochemistry. We provide a gene expression dataset that may be re-used to investigate corneal neovascularisation, and may also have implications in other contexts of inflammation-mediated angiogenesis.

Design Type(s): parallel group design, intervention design, in vivo design
Measurement Type(s): transcription profiling assay
Technology Type(s): DNA microarray
Factor Type(s): surgery
Sample Characteristic(s): Rattus norvegicus, cornea

Read the full article: A microarray whole-genome gene expression dataset in a rat model of inflammatory corneal angiogenesis 
Read the related article: Factors regulating capillary remodeling in a reversible model of inflammatory corneal angiogenesis